The ubiquitinylation and subsequent degradation of p53 is controlled by Mdm2, an oncogenic E3 ligase, or upon infection by virally encoded E3 ligases such as human papilloma virus E6 protein. p53 undergoes a number of post-translational modifications including conjugation with ubiquitin (Ub) and ubiquitin-like proteins (Ubl), such as SUMO and NEDD8, primarily at lysine residues located within its C-terminal domain. BIOMOL announces the release of a recombinant protein comprising the RING domain from Hdm2, the human homolog of Mdm2, which retains the E3 activity of the intact molecule, together with four antibodies specifically characterized for the detection of modified forms of p53.
Image: Lanes 1 & 3 - SUMO-modified p53. Lanes 2 & 4 - unmodified p53.
- Hdm2 catalytic RING domain (human, recombinant)
- PIAS1 (human, recombinant)
E3 Ligases for p53:
- p53, mouse mAb [clone EX-1]
- p53, mouse mAb [clone EX-2]
- p53, mouse mAb [clone EX-3]
- p53, mouse mAb [clone EX-4]
New p53 antibodies:
Characterized for the detection of Ub and Ubl-modified p53:
[Clone EX-1]. Recognizes a C-terminal epitope of p53. Will not detect C-terminally ubiquitinylated or SUMOylated p53 and is thus useful for discriminating modified from unmodified forms.
[Clone EX-2]. Recognizes an N-terminal epitope of p53, from a region devoid of lysines, making EX-2 ideally suited for detecting C-terminally Ub/Ubl-modified p53.
[Clones EX-3 and EX-4]. Both recognize an N-terminal epitope of p53 and are thus suitable for detecting C-terminally directed Ub/Ubl-modifications of p53.
- p53 proteins, activators, inhibitors and antibodies
- E1, E2, E3 & deconjugating enzymes
- Proteasome reagents
Related products:
- www.biomol.com/ubiquitons&zomes
Visit our Ubiquitons & Zomes Portal:
It is well established that amplification, mutation or misregulation of kinases contributes to many human diseases. To facilitate further development of new kinase inhibitors, BIOMOL offers over 120 active recombinant kinases and associated substrates for inhibitor screening or selectivity studies. All kinases and substrates are also available in bulk.
- Glucokinase (human, recombinant)
- SGK3 (human, recombinant)
- PKAc alpha (human, recombinant)
- TRKA (human, recombinant)
- TRKB (human, recombinant)
- PKC theta (human, recombinant)
New kinases:
- Serine/threonine kinases
- Tyrosine kinases
- Kinase substrates
- Kinase inhibitors
- Phosphospecific
antibodies
Related products:
Kinase Research Product Guide. A comprehensive guide detailing over 400 research products for kinase research, including enzymes, substrates, inhibitors and antibodies.Request a copy.
Provides a rich source of fatty acids for receptor de-orphaning, inhibitor screening, and high content screening. Compounds supplied in DMSO at 10 mM and aliquoted into deep-well plates at either 100 or 500 microlitres per well. Contains 68 fatty acids of varying saturation and chain length. Request compound listing and structure fileshere.
- Unsaturated and saturated fatty acids
- C10-24 chain length
- E and Z isomers
- Arachidonic acid
- gamma-Linolenic acid
- Linolenic acid
This library includes:
- Bioactive Lipid Library
- Endocannabinoid Library
- ICCB Known Bioactives Library
- Ion Channel Ligand Library
- Kinase Inhibitor Library
- Natural Products Library
- Neurotransmitter Library
- Nuclear Receptor Ligand Library
- Orphan Ligand Library
- Phosphatase Inhibitor Library
- Protease Inhibitor Library
- Rare Natural Products Library
Related products:
- PBA1, rabbit pAb
- PBF1, rabbit pAb
- Rpn12, rabbit pAb
- Rpt2, rabbit pAb
New antibodies to Arabidopsis proteasome subunits:
Related products:
BIOMOL offers over 75 antibodies to proteasome subunits:
- SerpinB6/PI6, mouse mAb
- SerpinB9/PI9, mouse mAb
- Zmpste24/FACE-1, rabbit pAb
New protease-related antibodies:
Related products:
Senior staff at BIOMOL were among the first to commercialize the peptide approach to antibody production and hold an unrivaled record of success in the production of protein-reactive antibodies. Let us apply our experience and proprietary expertise to the following services for you: project planning, antigen selection and synthesis, immunogen preparation, immunization, and antibody characterization. Find outmore.
