Bethyl
Laboratories, the polyclonal antibody specialists, announces the release of
our new PathProfiler™ Kits for the qualitative determination of protein
modification as determined by ELISA. Kits available include Histone
H3 and MCM2.
Each PathProfiler™
Kit includes a base module and the choice of two Detection Antibody
Modules. You can mix and match to fit your research requirements. Additional
Detection Modules are also available separately.
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Remember to use
your coupon book.
To request a coupon book, send
an email to Bethyl
Laboratories with your name and address. U.S. customers only.
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Detection of Total Histone H3 and Phospho Histone H3 (S10) by PathProfiler
ELISA (E300-001).
A549, HeLa, K562, and Jurkat cell lines were serum starved overnight. Cells
were subsequently serum stimulated and treated with 0.5 µg/ml of Calyculin
A at 37ºC, 5% CO2 for 15 minutes. Cells were lysed and screened using
PathProfiler Total Histone H3 and Histone H3 (S10) Detection Modules (E300-001A
and E300-001B,
respectively).
Detection of Total MCM2, Phospho MCM2 (S27), and Phospho MCM2 (S108) by
PathProfiler ELISA E300-002).
A549, HeLa, K562, and Jurkat cell lines were lysed with Cell Lysis Buffer.
Total protein concentration was determined and samples were diluted to 20
µg/ml in Dilution Buffer A and screened using PathProfiler Total MCM2,
Phospho MCM2 (S27), and Phospho MCM2 (S108) Detection Modules (E300-002A,
E300-002B,
and E300-002C,
respectively).
