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Bethyl Laboratories, Inc.

Polyclonal Antibodies Validated for IHC and ICC

September 2009

Bethyl Laboratories' expanding portfolio of over 400 antibodies and reagents for IHC includes a complete line of accessory kits and reagents. Our IHC accessory products are designed to simplify and enhance your experience with our IHC antibodies. Our Immunohistochemistry Accessory Kit includes most of the reagents required for IHC, including, Epitope Retrieval Buffer Concentrate-Reduced pH, Ready-To-Use IHC Blocking Reagent, Ready-To-Use IHC Antibody Diluent, anti-Rabbit IHC Antibody Concentrate, IHC Wash Solution Concentrate, DAB Substrate, Ready-To-Use IHC Hematoxylin, Ready-To-Use IHC Bluing Reagent, and Epitope Retrieval Buffer Concentrate-High pH. We also offer three sizes of Normal Goat Serum (50 ml, 100 ml, and 250 ml).

14-3-3 Sigma
4EBP1
4E-T/eIF4E-T
53BP1
53BP1 (S25)
ABCB9

ABI1
ACINUS/ACN1
ADNP
AF9
AKAP1
AKAP8/AKAP95
AKAP8L/HA95
ALMS1
AMPK alpha 1
Anillin
ANKS3
APC
ARID1B
Artemis -
NEW!
ASC2
ASH2
ASPM
ASPP2
ATM
ATRX
Aurora A
Aurora A (T288)
Aurora B
BAAT1
BAF53A
BAF57/SMARCE1
BCAS2
Bcl11b
BCR -
NEW!
BHC110/LSD1
BID
BIRC6/Apollon
BLAP75
BRCA1
BRD4
BRD8
BRF1
BTF
Bub3
CA150
cAbl -
NEW!
CAD
CAF-1 p60
CamK4
Caper
CARM1
CBP
CBX3
CBX5
CC2D1A -
NEW!
CCAR1
CD31
CDC7 -
NEW!
CDC20
CDC25c
CDK2
CDK11
CDK5RAP2
CDT1
CDX2
CENP-B
CENP-F/Mitosin
CEP131/AZ1
CEP170
CEP290
CEP78
CHD3
CHD8
Chk1
Chk1 (S317)
Chk2
CIP2A
CKII alpha
CoAA
COBRA1
CoREST
Coronin 1
Coronin 2
CPSF59
CPSF68
CPSF73
CPSF160
CRABP2
CREB
CRM1
CRSP1/TRAP220
CSE1
CSN1
CSN2
CSN3
CSN5
CSTF50
CSTF64
CSTF77
CTBP1
CTCF
CTF18
CTR9
Cul3
Cul4a
Cul7
Cyclin E1
DBC1/p30 DBC
DDB1
DDX1
DDX5
DDX17
DDX21
DDX24
DDX46
DDX54
DEK
DFF45/DFFA
DHX8
DHX9
DHX15
DHX38
DMWD
DNA Ligase 1
DNA-PKcs
DNL3
DRIL1

Drosha
E1B-AP5

EDD1
eEF2
eEF2 Kinase
EFTUD2/SNRP116
EGFR
EHMT1
EMSY -
NEW!
EPS15

ErbB2
ERCC3
ERF
Estrogen Receptor Alpha
EWS
EZH2 (S21)
FAS
FBW7
Fen1
Filamin A
Filamin B
FIP1
FKBP4/FKBP52
FKBP5/FKBP51
FLASH
Flightless-1
FLRF/RNF41
FOG1/ZFPM1
FoxK1
FoxK2
FUS
G6PD
gamma-H2AX
GAPDH
Geminin
GNL3
GTF2I/TFII-I
GTF2IRD/TFII-IRD1
GTF3C1/TFIIIC220
GTF3C2/TFIIIC110
GTF3C3/TFIIIC102
GTF3C4/TFIIIC90
GTF3C5/TFIIIC63
H2AX
HDAC1
HDAC2
HDAC7
HdmX/MDM4
Histone H3 (S10)
Histone H4
HMG2a
HMGN3
hnRNP-H
hnRNP-K
hnRNP-U
HPIP
hSET1
HURP/DLG7
IKK-alpha -
NEW!
ILK
INCENP
INT4
INT7 -
NEW!
INT8 -
NEW!
INT11
INTS9
IQGAP1 -
NEW!
IRF3
IRS1
IRS2
Jarid1B
JMJD1B
JMJD1C
JMJD2A
KANK2/SIP
KAP-1
KAP-1 (S824)
Ki67
KIAA0082 -
NEW!
KPNA3
KPNA4
LMO1
MAD1
MAD2 -
NEW!
MafA
MafB
MAGED2
Matrin 3
MBD2
MBD4
MCAF
MCM2
MCM2 (S108)
MCM2 (S27)
MCM2 (S40/S41)
MCM2 (S41)
MCM2 (S53)
MCM3
MCM4
MCM5
MCM6
MDC1
MED12
MED18
METTL3/MT-A70
MLH3
Mre11
MSH2
MSH6
MTA1
MYBBP1A
Nanog
NBS1
NCOA62
NELFA
NELFE
NF2 isoform 1
NIF1
NMI
NONO
NRBF2
NSD1
NUCKS
Nucleolin
NUMA
NUP35
NUP50
NUP153
NUP214
p14ARF
p53
p66beta/GATAD2B
p73
p300
Paf1
PALB2
Parafibromin
PARP1
PARP10
PBEF
PC4
PCNA
PCNA (Y211)
Pds5B
PELP1/MNAR
Pericentrin/Kendrin

Pescadillo

PGC-1

PHF6
PHF8
PHLPP
PLK1
PLRG1 -
NEW!
PML
PNK1
PNUTS
Pol Lambda
PolD3/p66
PolE3/p17
PPM1G
PPP4C
PRKRIR
PRMT1
PRMT5
PRP19/PSO4
PSF/SFPQ
PTEN
PTPN12/PTP-PEST
PUS1
Rad6
Rad9
Rad50
RanBP3
RbBP4
RbBP5
RbBP7
RBM9
RBM12
RBM16
RBM25/RED120
RBM26
RBM27
RCC2
RCH1/KPNA2
RCOR3
REA
RecQ1
REST
Reticulocalbin-1
RFC3 -
NEW!
RIF1
RNA Polymerase II (S2)
RNA Polymerase II (S5)
RNF20
RNF40
RPA32 -
NEW!
RPA32 (S4/S8) -
NEW!
RPA32 (S33) -
NEW!
RPA70
 - NEW!
RPL26
Rtf1
SAFB1
SAFB2
SART3/TIP110
SF3a120/SAP114
SF3b145/SAP145
SF3b155/SAP155
SFRS14
Sin1
Sin3A
SMAR1/BANP
SMARCA1/SNF2L
SMARCB1/SNF5
SMARCC2/BAF170
SMC1
SMC1 (S966)
SMC3
SMC3 (S1083)
SMC6
SMG1
Sp1
SPTAN1/Alpha II-spectrin
SPTBN1
SRA
SRC2
SSB1 -
NEW!
SSH3
STAT6
STUB1/CHIP
SUPT5H
SUPT6H
TAF6
TAFII68
TBLR1
TCEB3
TDP1
TFII-I
THRAP3/TRAP150
TIF1 Alpha/TRIM24
Timeless
Topo II Alpha
Topo II Beta
TPR
TReP-132/TRERF1
TRIM3/BERP
TRIM33/TIF1gamma
TRIP1/SUG1
Tyro3 -
NEW!
UACA
UBCH7
UBE1
Ubiquitin -
NEW!
USP1
USP3
USP5/IsoT
USP7
USP10
USP28
USP47
VCP
Vimentin
WDFY3
WDR77/MEP50
WDR91
WRN -
NEW!
WRNIP1
WTAP
XLF
XPA
XPF/ERCC4
XRCC1 (S461)
XRCC1 (S485/T488)
XRCC1 (S518/T519/T523)
XRN2
ZBTB40 -
NEW!
ZC3H11A
ZCCHC8
ZEB1 -
NEW!
ZFP106
ZHX1
ZHX3
ZNF261
ZNF295
ZNF318/TZF
ZNF326
ZWINT-1

 



Detection of Human BCR by IHC-IF


Sample:
FFPE section of human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene).  Antibody: Affinity purified rabbit anti-BCR (Cat. No. IHC-00406) used at a dilution of 1:250.  Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte Plus 555 (A120-501E) used at a dilution of 1:100.

Hilyte is a trademark of AnaSpec, Inc.



Detection of Human Aurora B
by ICC-IF.

Sample: NBF-fixed HeLa Cells. Antibody: Affinity purified rabbit anti-Aurora B (Cat. No. IHC-00003) used at a dilution of 1:100.  Detection: Goat anti-rabbit IgG (h&l) FITC (Cat. No. A120-101F) used at a dilution of 1:100.



Detection of Human NUP153 by ICC-IF. 

Sample: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-NUP153 (Cat. No. IHC-00366) used at a dilution of 1:250. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte Plus™ 555 (A120-501E) used at a dilution of 1:100.

Search for Antibodies by Research Area
Bethyl Laboratories' portfolio of polyclonal antibodies commercially available includes over 3500 polyclonal antibodies to over 1300 proteins. We test our antibodies in a wide range of applications including ELISA, western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry. We have recently validated many of our antibodies for flow cytometry and ChIP. Our antibody range also includes secondary antibodies, loading controls and antibodies to epitope tags.

Apoptosis | Cell Cycle | Cell Division | Chromatin Remodeling | Cytoskeletal Organization
DNA Damage/Repair | Histone Modification | Hypoxia | Kinase/Phosphatase | Neurobiology
Nuclear Import/Export | Nuclear Receptor Coactivators | Phospho-specific
RNA Processing | Signal Transduction | Telomeric Maintenance | Transcription Factor/Regulator Translation Control | Transport/Trafficking | Tumor Suppressors/Oncoproteins | Ubiquitination

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