The SMARCs
(SWI/SNF-related, matrix-associated, actin-dependent
regulators of chromatin), and BAFs (BRG1-associated
factors), have been identified as components of the
mammalian SWI/SNF-like chromatin-remodeling protein
complexes. These multi-protein complexes are proposed to
function as ATP-driven motors that translocate along DNA
and destabilize nucleosomal structures to facilitate
transcription factor binding. Two fractions of
SWI/SNF-like complexes, SWI/SNF-A (BAF) and SWI/SNF-B
(PBAF), have been isolated. The SWI/SNF-A (BAF) complex
is defined by the presence of a BAF250/ARID1A subunit
and either a SMARCA4/Brg1 or SMARCA2/Brm subunit, while
the SWI/SNF-B (PBAF) complex lacks the BAF250/ARID1A
subunit and contains a SMARCA4/Brg1 subunit and a
polybromo/BAF180 subunit. In addition to these defining
subunits, the SWI/SNF-A (BAF) and SWI/SNF-B (PBAF)
complexes possess multiple subunits of other SMARCs
and/or BAFs that are present in various combinations to
provide the mammalian cell with a functional variety of
SWI/SNF-like chromatin-remodeling complexes. This
growing family of interacting proteins has also been
shown to associate with the N-CoR (nuclear receptor
co-repressor) deacetylase complex and various nuclear
transcription factors. Current information indicates an
important role for these complexes in the regulation of
cell growth and proliferation. Future studies will
likely demonstrate that the SWI/SNF proteins are
important to many aspects of development and
tumorigenesis.
Bethyl Laboratories' current
portfolio of antibodies to xxx, includes,
ARID1B,
BAF250/ARID1A,
BAF53A,
SMARCA1/SNF2L,
SMARCA2/BRM,
SMARCA3,
SMARCA4/BRG1,
SMARCB1/SNF5,
SMARCC1/BAF155,
SMARCC2/BAF170,
SMARCE1/BAF57, and
SNF2h/ISWI.
