Bethyl Laboratories, Inc.

Bethyl Laboratories, Inc.

Antibodies to Proteins Involved in
Histone Acetylation and Deacetylation

February 2010

Overview

Histone modification is central to chromatin architecture and the modulation of transcription. Histone deacetylases (HDACs) and histone acetylases (HATs) are chromatin-modifiying enzymes that catalyze the removal and addition of acetyl groups from acetyl-lysine residues on the N-terminal extensions of core histones. Acetylated histones are a key feature of actively transcribed genes. Histone acetylation and transcriptional activation is counteracted by deacetylation which results in transcriptional repression. Aberrant histone acetylation has been observed in human cancers. Recently it has become recognized that inhibiting chromatin-modifying enzymes such as HDACs may provide an avenue for the development of anti-cancer agents. Although this avenue has promise, the development of such agents still requires a more detailed analysis and better understanding of the complex regulatory mechanisms of histone acetylation.

Selected Reviews
1. P. Gallinari et al., "HDACs, Histone Deacetylation and Gene Transcription: From Molecular Biology to Cancer Therapeutics," Cell Res. 17, no. 3 (2007): 195-211.

2. J. K. Choi and L. J. Howe, "Histone Acetylation: Truth of Consequences?," Biochem.Cell Biol. 87, no. 1 (2009): 139-150.

Bethyl Laboratories' Portfolio
Baf53A BCoR CBP CBX5 CHD3 CHD4/Mi2 beta CoAA CtIP FoxP3 HDAC1 HDAC2 HDAC3 HDAC6 HDAC7 KPNA2/RCH1 MBD3 MTA2 MYST1/MOF PGC-1 Prohibitin Rad9 RbBP4 RbBP7 SATB2 SDS3 SET Sirt1 Sirt2 Sirt6 Sp1 TBLR1 Topo II Alpha Topo II Beta	Detection of Human HDAC6 by WB and IP. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HDAC6 antibody A301-341A used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. HDAC6 was also immunoprecipitated by rabbit anti-HDAC6 antibody A301-342A, which recognizes a downstream epitope. For detecting immunoprecipitated HDAC6, ReliaBLOT Reagents and Procedures (Cat. No. WB120) were used. Detection: Chemiluminescence with exposure times of 5 minutes (A) or 30 seconds (B).	Detection of Human Topo II beta by WB and IP. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Topo II Beta antibody A300-950A used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B). Topo II Beta was also immunoprecipitated by rabbit anti-Topo II Beta antibodies A300-949A and BL4193, which recognize upstream epitopes. For detecting immunoprecipitated Topo II beta, ReliaBLOT Reagents and Procedures (Cat. No. WB120) were used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Search for Antibodies by Research Area
Bethyl Laboratories' portfolio of polyclonal antibodies commercially available includes over 3500 polyclonal antibodies to over 1300 proteins. We test our antibodies in a wide range of applications including ELISA, western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry. We have recently validated many of our antibodies for flow cytometry and ChIP. Our antibody range also includes secondary antibodies, loading controls and antibodies to epitope tags.

Bethyl Laboratories, Inc. - P.O. Box 850 - Montgomery, TX 77356
Phone: 1-800-338-9579 - Fax: 1-866-597-6105