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E-Newsletter
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January 21, 2008
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Featured
Products - Antibodies to RRM-containing Proteins
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The RNA recognition motif (RRM) is a highly conserved
RNA-binding domain and is present in one, two, or multiple copies in
RNA-binding proteins.
The RRM consists of a 90 amino acid domain that contains two consensus
sequences termed ribonucleoprotein (RNP) domains. The first RNP is an eight
amino acid sequence (RNP1) centrally located in the 90 amino acid RRM,
while the second is a six amino acid sequence (RNP2) located at the
N-terminus of the RRM. Proteins with an RRM motif are abundant and found
mostly in eukaryotes. They are diverse in structure and function and are
involved in all post-transcriptional processes such as pre-mRNA processing,
splicing, RNA editing, mRNA export, translational regulation, and mRNA
stability and degradation. In recent years, the RRM has been shown to also
be involved in protein-protein interactions. Structural studies of RRM
domains and the complexes they form with RNA and proteins have given some
insight into the determinants of their binding specificity. Current
information on RRM-containing proteins reveals that the role of the RRM in
the cell may extend beyond a simple component of RNA recognition. Although
a multitude of proteins bearing an RRM motif have been identified, the
specific functions of many remain uncharacterized. Further biochemical and
structural studies are needed to understand the evolution and functional
diversity of RRM-containing proteins.
Bethyl Laboratories' portfolio of antibodies to proteins containing the RNA
recognition motif, includes Caper,
CoAA,
CSTF64,
EWS,
FUS,
hnRNP-H,
hSET1,
Matrin
3, NONO,
Nucleolin,
PGC-1,
PSF/SFPQ,
RBM6,
RBM8a,
RBM9,
RBM10,
RBM12,
RBM15,
RBM16,
RBM25/RED120,
RBM26,
SAFB1,
SAFB2,
SHARP,
and TAFII68.
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Detection of Human RBM26 by Immunohistochemistry.
Sample: FFPE
section of human prostate carcinoma. Antibody: Affinity purified rabbit
anti-RBM26 (Cat. No. IHC-00245) used at a
dilution of 1:250. Detection: DAB staining using Immunohistochemistry
Accessory Kit (Cat. No. IHC-101).
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New Polyclonal
Antibodies
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Detection of Human ACF1/BAZ1A by Western Blot and
Immunoprecipitation.
Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP
loaded) from HeLa cells. Antibodies: Affinity purified rabbit
anti-ACF1/BAZ1A antibody A301-318A used for
WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.
ACF1/BAZ1A was also immunoprecipitated by rabbit anti-ACF1/BAZ1A antibody A301-319A, which
recognizes a downstream epitope. For blotting immunoprecipitated
ACF1/BAZ1A, ReliaBLOT® Reagents and Procedures (Cat. No. WB120) were used.
Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
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Detection of Human and Mouse PSF/SFPQ by Western Blot
(h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50
mcg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 mcg)
cells. Antibodies: Affinity purified rabbit anti-PSF/SFPQ antibody A301-322A used for
WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.
PSF/SFPQ was also immunoprecipitated by rabbit anti-PSF/SFPQ antibodies A301-320A and
A301-321A, which recognize upstream epitopes. For blotting
immunoprecipitated PSF/SFPQ, ReliaBLOT® Reagents and Procedures (Cat. No. WB120) were used.
Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1
second (B).
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New
IHC Antibodies
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Detection of Human RBBP4/RbAp48 by Immunohistochemistry. Sample: FFPE section of human skin squamous cell
carcinoma. Antibody: Affinity purified rabbit anti-RBBP4/RbAp48 (Cat. No. IHC-00242) used at a
dilution of 1:500. Detection: DAB staining using Immunohistochemistry
Accessory Kit (Cat. No. IHC-101).
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Detection of Human TRIM3/BERP by Immunohistochemistry. Sample: FFPE section of human anaplastic thyroid
carcinoma. Antibody: Affinity purified rabbit anti-TRIM3/BERP (Cat. No. IHC-00243) used at a
dilution of 1:250. Detection: DAB staining using Immunohistochemistry
Accessory Kit (Cat. No. IHC-101).
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Current
Citations
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ASPM
(IHC-0058)
Mutations
in pericentrin cause Seckel syndrome with defective ATR-dependent DNA
damage signaling
Elen Griffith, Sarah Walker, Carol-Anne Martin, Paola Vagnarelli, Tom
Stiff, Bertrand Vernay, Nouriya Al Sanna, Anand Saggar, Ben Hamel, William
C Earnshaw, Penny A Jeggo, Andrew P Jackson, Mark O'Driscoll
Nature
Genetics (23 Dec 2007)
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Set1, MLL, MLL2/Trx2
p38 MAPK
signaling regulates recruitment of Ash2L-containing methyltransferase
complexes to specific genes during differentiation
Shravanti Rampalli, LiFang Li, Esther Mak, Kai Ge, Marjorie Brand, Stephen
J Tapscott, F Jeffrey Dilworth
Nature
Structural & Molecular Biology 14, 1150-1156 (01 Dec 2007)
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RPA2-pS4/S8, Chk1, SMC1, SMC1-S966, SMC3-pS1083
Human CtIP
promotes DNA end resection
Alessandro A. Sartori, Claudia Lukas, Julia Coates, Martin Mistrik, Shuang
Fu, Jiri Bartek, Richard Baer, Jiri Lukas, Stephen P. Jackson
Nature
450, 509-514 (22 Nov 2007)
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