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ANTIBODIES TO KINESINS
The kinesins (KIFs) make up a large superfamily of motor proteins that function in intracellular transport along microtubules. Kinesin activity has been linked to various cellular functions such as organelle and vesicle transport, mitotic spindle formation, chromosome segregation, and cytokinesis. Structurally, all kinesins contain a motor domain with microtubule and nucleotide binding sites that utilize ATP to target cargo along microtubule filaments. The kinesin motor proteins have been implicated in human disease.A mutation in the KIF1B locus is associated with Charcot-Marie-Tooth disease (CMT2A) which is characterized by a progressive dysfunction of peripheral neurons and a lack of synaptic vesicle transport in nerve endings and axons. Over forty members of the KIF family have been identified and well characterized. However, when one considers the multitude of cellular tasks involving the intracellular transport of cargo, many questions concerning KIF regulation remain unanswered.

Please follow the links in the table for specific product information our our current selection of antibodies to KIF family members.

Detection of Human KIF1C/LTXS1 by WB and IP. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-KIF1C/LTXS1 antibody A301-070A used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. KIF1C/LTXS1 was also immunoprecipitated by rabbit anti-KIF1C/LTXS1 antibodies A301-069A, A301-071A and A301-072A, which recognize other epitopes. For blotting immunoprecipitated KIF1C/LTXS1, ReliaBLOT^® Reagents and Procedures (Cat. No. WB120) were used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

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A. Detection of Human KIF13A by WB and IP. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-KIF13A antibody A301-077A used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. KIF13A was also immunoprecipitated by rabbit anti-KIF13A antibody A301-078A, which recognizes a downstream epitope. For blotting immunoprecipitated KIF13A, ReliaBLOT^® Reagents and Procedures (Cat. No. WB120) were used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).



Detection of Human KIF4A by WB and IP. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-KIF4A antibody A301-074A used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. KIF4A was also immunoprecipitated by rabbit anti-KIF4A antibody A301-073A, which recognizes an upstream epitope. For blotting immunoprecipitated KIF4A, ReliaBLOT^® Reagents and Procedures (Cat. No. WB120) were used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

RECENT CITATIONS ...

A functional genomic screen identifies a role for TAO1 kinase in spindle-checkpoint signalling.
Viji M. Draviam, Frank Stegmeier, Grzegorz Nalepa, Mathew E. Sowa, Jing Chen, Anthony Liang, Gregory J. Hannon, Peter K. Sorger, J. Wade Harper, Stephen J. Elledge
...rabbit anti-BubR1 (kind gift from H. Yu, Southwestern Medical School, Dallas, TX), rabbit anti-TAO1 (Bethyl Laboratories, Montgomery, TX) were used. For immunoprecipitation reactions, cells were lysed in CHAPS lysis...
Nature Cell Biology 9, 556 - 564 (01 May 2007).

The SMC5/6 complex maintains telomere length in ALT cancer cells through SUMOylation of telomere-binding proteins.
Patrick Ryan Potts, Hongtao Yu
...anti-SCC1 (Bethyl Laboratories, A300-080A), anti-SMC1 (Bethyl Laboratories, A300-055A), anti-SMC5 (Bethyl Laboratories, A300-236A), anti-SMC6 (Bethyl Laboratories, A300-A237A) and anti-TRF2...
Nature Structural & Molecular Biology 14, 581 - 590 (01 Jul 2007).

Regulation of ATR-dependent pathways by the FHA domain containing protein SNIP1.
K C Roche, S Rocha, C P Bracken, N D Perkins
...and anti-phospho-SMC1 serine 966 and anti-SMC1 antibodies; Bethyl Laboratories, Montgomery, TX, USA)...
Oncogene 26, 4523 - 4530 (05 Jul 2007).

Detection of Human EG5/KIF11 by WB and IP. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-EG5/KIF11 antibody A301-075A used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. EG5/KIF11 was also immunoprecipitated by rabbit anti-EG5/KIF11 antibody A301-076A, which recognizes a downstream epitope. For blotting immunoprecipitated EG5/KIF11, ReliaBLOT^® Reagents and Procedures (Cat. No. WB120) were used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).