Antibodies to DExD/H Proteins

Enzyme profiling is an important step in the drug discovery process where a lead compound is tested
for specificity against a panel of similar enzymes. As an example of the size of the enzyme profiling market,
it is estimated that $10 to $40 million is spent on protease profiling annually.

BIOMOL offers a number of enzyme profiling kits and assortment packs that facilitate rapid and economical
screening of compounds for specificity against focused enzyme panels.
Currently, BIOMOL offers panels of MMPs, phosphatases, ubiquitin-conjugating enzymes and caspases.
Each kit contains a panel of similar enzymes and the MMP Inhibitor Profiling Kits each contain a complete set
of reagents to screen MMP activity.
These kits are an economical way to screen a panel of enzymes without having to clone, express and develop
assays for multiple enzymes.
In addition to these enzyme panels, BIOMOL can put together custom panels from over 400 recombinant
enzymes in our product line including over 110 kinases.

For further information www.BIOMOL.com

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ANTIBODIES TO KINESINS

By Eric McIntosh, Ph.D.: 21.08.07 in Drug discovery research

The kinesins (KIFs) make up a large superfamily of motor proteins that function in intracellular transport along microtubules. Kinesin activity has been linked to various cellular functions such as organelle and vesicle transport, mitotic spindle formation, chromosome segregation, and cytokinesis.

Structurally, all kinesins contain a motor domain with microtubule and nucleotide binding sites that utilize ATP to target cargo along microtubule filaments. The kinesin motor proteins have been implicated in human disease.A mutation in the KIF1B locus is associated with Charcot-Marie-Tooth disease (CMT2A) which is characterized by a progressive dysfunction of peripheral neurons and a lack of synaptic vesicle transport in nerve endings and axons.

Over forty members of the KIF family have been identified and well characterized. However, when one considers the multitude of cellular tasks involving the intracellular transport of cargo, many questions concerning KIF regulation remain unanswered.

Please follow the links in the table for specific product information our our current selection of antibodies to KIF family members.

KIF1B	KIF2A	KIF11/EG5	KIF14
KIF1C/LTXS1	KIF4A	KIF13A	KIF18A

Figure:
Kinesin
Detection of Human KIF1C/LTXS1 by WB and IP. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-KIF1C/LTXS1 antibody A301-070A used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. KIF1C/LTXS1 was also immunoprecipitated by rabbit anti-KIF1C/LTXS1 antibodies A301-069A, A301-071A and A301-072A, which recognize other epitopes. For blotting immunoprecipitated KIF1C/LTXS1, ReliaBLOT^® Reagents and Procedures (Cat. No. WB120) were used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

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Colon cancer update

By Tom Vendelbo: 10.01.07 in Drug discovery research

Our partner, Bethyl Laboratories, offers an extensive selection of antibodies to proteins involved in the DNA repari process. Here is the most recent product update in this area.

Quote from January Product Update:

Mutations in the MSH6 gene have been reported in about 10 percent of hereditary nonpolyposis colon cancer (HNPCC) families that have an identified gene mutation. All of these mutations cause the production of an abnormally short, nonfunctional MSH6 protein or a partially active version of the protein. When the MSH6 protein is absent or working inadequately the number of mistakes that are left unrepaired during cell division increases substantially.

If the cells continue to divide, errors accumulate in DNA; the cells become unable to function properly and may form a tumor in the colon or another part of the body - particularly the endometrium.

Link to Bethyl’s selection of DNA Damage/Repair antibodies.

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