ANTIBODIES TO KINESINS
By Eric McIntosh, Ph.D.: 21.08.07 in Drug discovery research
The kinesins (KIFs) make up a large superfamily of motor proteins that function in intracellular transport along microtubules. Kinesin activity has been linked to various cellular functions such as organelle and vesicle transport, mitotic spindle formation, chromosome segregation, and cytokinesis.
Structurally, all kinesins contain a motor domain with microtubule and nucleotide binding sites that utilize ATP to target cargo along microtubule filaments. The kinesin motor proteins have been implicated in human disease.A mutation in the KIF1B locus is associated with Charcot-Marie-Tooth disease (CMT2A) which is characterized by a progressive dysfunction of peripheral neurons and a lack of synaptic vesicle transport in nerve endings and axons.
Over forty members of the KIF family have been identified and well characterized. However, when one considers the multitude of cellular tasks involving the intracellular transport of cargo, many questions concerning KIF regulation remain unanswered.
Please follow the links in the table for specific product information our our current selection of antibodies to KIF family members.
| KIF1B | KIF2A | KIF11/EG5 | KIF14 |
| KIF1C/LTXS1 | KIF4A | KIF13A | KIF18A |
Figure:
Detection of Human KIF1C/LTXS1 by WB and IP. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-KIF1C/LTXS1 antibody A301-070A used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. KIF1C/LTXS1 was also immunoprecipitated by rabbit anti-KIF1C/LTXS1 antibodies A301-069A, A301-071A and A301-072A, which recognize other epitopes. For blotting immunoprecipitated KIF1C/LTXS1, ReliaBLOT® Reagents and Procedures (Cat. No. WB120) were used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).